Plasmid_Backbone

Part:BBa_K563053:Design

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal NheI site found at 3839
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
    Illegal NotI site found at 576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal BamHI site found at 519
    Illegal XhoI site found at 583
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 595
    Illegal SpeI site found at 525
    Illegal SpeI site found at 5851
    Illegal PstI site found at 555
    Illegal PstI site found at 3632
    Illegal PstI site found at 4120
    Illegal NgoMIV site found at 5709
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1996
    Illegal BsaI.rc site found at 2942
    Illegal SapI site found at 913
    Illegal SapI site found at 3094


Design Notes

We use this vector for expressing mutant Tor2. In order to operate easily, we separate Tor2 into three parts. So we reconstruct the pYE by adding specific restriction sites for linking.


Source

we bought it from invitrogen

References